\u751f\u7269\u8cc7\u6e90\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u5ba4\u3067\u306f(i) \u5bc4\u751f\u866b\u5b66\u3001\u611f\u67d3\u75c7\u3084\u514d\u75ab\u5b66\u306b\u95a2\u3059\u308b\u7814\u7a76\u3084\u3001(ii) \u30de\u30a6\u30b9\u3092\u7528\u3044\u305f\u751f\u6b96\u751f\u7269\u5b66\u306e\u7814\u7a76\u3001(iii) \u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u306e\u63a8\u9032\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001(iv) \u30d2\u30c8\u306e\u751f\u6b96\u88dc\u52a9\u533b\u7642\u3078\u306e\u8ca2\u732e\u3092\u6700\u7d42\u76ee\u7684\u3068\u3057\u3066\u3001\u30de\u30a6\u30b9\u521d\u671f\u80da\u64cd\u4f5c\u6280\u8853\uff08\u4f8b\uff1a\u4f53\u5916\u53d7\u7cbe\u3001\u53d7\u7cbe\u5375\u51cd\u7d50\u3001\u7cbe\u5b50\u51cd\u7d50\u7b49\uff09\u306e\u5411\u4e0a\u3092\u8a66\u307f\u3066\u3044\u307e\u3059\u3002\u672c\u6280\u8853\u306f\u3001\u8cb4\u91cd\u306a\u30de\u30a6\u30b9\u7cfb\u7d71\u306e\u4fdd\u5b58\u3084\u9069\u5207\u306a\u52d5\u7269\u98fc\u80b2\u74b0\u5883\u7dad\u6301\u306b\u3082\u8ca2\u732e\u3057\u307e\u3059\u3002<\/p>\n
\u6559\u80b2\u9762\u3067\u3082\u3001\u5b66\u90e8\u751f\u304a\u3088\u3073\u5927\u5b66\u9662\u751f\u3078\u306e\u5b9f\u9a13\u52d5\u7269\u5b66\u3068\u52d5\u7269\u5b9f\u9a13\u502b\u7406\u306e\u6559\u80b2\u3092\u62c5\u5f53\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n
<\/p>\n
\u301c\u300e\u5375\u5b50\u3063\u3066\u3069\u3046\u3084\u3063\u3066\u3067\u304d\u308b\u306e\uff1f\u300f\u301c\u3000\u975e\u5e38\u306b\u30b7\u30f3\u30d7\u30eb\u306a\u7591\u554f\u3067\u3059\u304c\u3001\u305d\u306e\u8a73\u3057\u3044\u4ed5\u7d44\u307f\u306f\u308f\u304b\u3089\u306a\u3044\u3053\u3068\u3060\u3089\u3051\u3067\u3059\u3002\u5375\u5b50\u306e\u8d77\u6e90\u3068\u306a\u308b\u59cb\u539f\u751f\u6b96\u7d30\u80de\u304c\u306f\u3058\u3081\u3066\u51fa\u73fe\u3059\u308b\u306e\u306f\u80ce\u4ed4\u671f\u3067\u3001\u305d\u3053\u304b\u3089\u975e\u5e38\u306b\u9577\u3044\u6642\u9593\u3092\u304b\u3051\u3066\u5375\u5de3\u306e\u4e2d\u3067\u53d7\u7cbe\u53ef\u80fd\u306a\u5375\u5b50\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u305d\u306e\u904e\u7a0b\u3067\u306f\u5375\u6bcd\u7d30\u80de\u7cfb\u5217\u3078\u306e\u5206\u5316\u30fb\u5375\u30b5\u30a4\u30ba\u306e\u6210\u9577\u30fb\u6e1b\u6570\u5206\u88c2\u30fb\u6bcd\u6027\u30a8\u30d4\u30b2\u30ce\u30e0\u306e\u7372\u5f97\u3068\u3044\u3063\u305f\u591a\u69d8\u306a\u73fe\u8c61\u304c\u9023\u7d9a\u7684\u306b\u8d77\u3053\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u73fe\u8c61\u304c\u3069\u308c\u304b\u3072\u3068\u3064\u3067\u3082\u6b20\u3051\u3066\u3057\u307e\u3046\u3068\u3001\u500b\u4f53\u767a\u751f\u3092\u652f\u6301\u3067\u304d\u308b\u5b8c\u5168\u306a\u5375\u306b\u306a\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u3053\u306e\u8907\u96d1\u306a\u5375\u5f62\u6210\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u7406\u89e3\u3057\u3001\u4e0a\u8a18\u306e\u7591\u554f\u306b\u7b54\u3048\u308b\u3079\u304f\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u79c1\u305f\u3061\u306f\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u3092\u5207\u308a\u53e3\u3068\u3057\u3066\u5375\u5f62\u6210\u6a5f\u69cb\u3092\u7406\u89e3\u3057\u3088\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u3002\u3072\u3068\u53e3\u306b\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u3068\u8a00\u3063\u3066\u3082\u3001\u5375\u5b50\u306e\u4e2d\u3067\u306f\u69d8\u3005\u306a\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u6a5f\u69cb\u304c\u50cd\u3044\u3066\u3044\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u30b2\u30ce\u30e0DNA\u306b\u30e1\u30c1\u30eb\u57fa\u3092\u4ed8\u4e0e\u3059\u308bDNA\u30e1\u30c1\u30eb\u5316\u3084\u30d2\u30b9\u30c8\u30f3\u30c6\u30fc\u30eb\u306e\u5316\u5b66\u4fee\u98fe\u304c\u305d\u306e\u4ee3\u8868\u7684\u306a\u3082\u306e\u3067\u3059\u3002<\/p>\n
DNA\u30e1\u30c1\u30eb\u5316\u306b\u3064\u3044\u3066\u306f\u3001DNA\u30e1\u30c1\u30eb\u57fa\u8ee2\u79fb\u9175\u7d20\u907a\u4f1d\u5b50\u7fa4\u3092\u7570\u6240\u7684\u306b\u767a\u73fe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u3092\u7528\u3044\u305f\u7814\u7a76\u3092\u9032\u3081\u3066\u304d\u307e\u3057\u305f(Sasaki et al., Mol Reprod Dev 2019)\u3002\u3053\u306e\u7814\u7a76\u306b\u3088\u3063\u3066\u3001DNA\u30e1\u30c1\u30eb\u57fa\u8ee2\u79fb\u9175\u7d20\u306e\u5b58\u5728\u306e\u307f\u3067\u306f\u5b8c\u5168\u306aDNA\u30e1\u30c1\u30eb\u5316\u3001\u3059\u306a\u308f\u3061\u3001\u5b8c\u5168\u306a\u6bcd\u6027\u30a8\u30d4\u30b2\u30ce\u30e0\u306e\u7372\u5f97\u306b\u306f\u81f3\u3089\u306a\u3044\u3053\u3068\u3001\u3055\u3089\u306b\u3001\u5375\u306f\u4f53\u7d30\u80de\u7cfb\u8b5c\u3068\u306f\u7570\u306a\u308b\u72ec\u7279\u306a\u30af\u30ed\u30de\u30c1\u30f3\u69cb\u9020\u3092\u3068\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u3053\u306e\u5375\u7279\u6709\u306a\u30af\u30ed\u30de\u30c1\u30f3\u69cb\u9020\u306e\u8a73\u7d30\u306f\u8fd1\u5e74\u306e\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u306b\u3088\u3063\u3066\u6025\u901f\u306b\u89e3\u660e\u3055\u308c\u3066\u304d\u3066\u3044\u307e\u3059\u3002\u73fe\u5728\u306f\u5375\u6bcd\u7d30\u80de\u3067\u3069\u306e\u3088\u3046\u306b\u3057\u3066\u30e6\u30cb\u30fc\u30af\u306a\u30af\u30ed\u30de\u30c1\u30f3\u69cb\u9020\u304c\u5f62\u4f5c\u3089\u308c\u308b\u306e\u304b\u306b\u7740\u76ee\u3057\u3066\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u3053\u306e\u7591\u554f\u306b\u7b54\u3048\u308b\u305f\u3081\u3001\u4f53\u5185 (in vivo)\u3068\u4f53\u5916 (in vitro)\u306e\u53cc\u65b9\u304b\u3089\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002In vivo\u304b\u3089\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u3068\u3057\u3066\u306f\u3001CRISPR-Cas\u3092\u57fa\u76e4\u3068\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u7528\u3044\u305f\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3084\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a4\u30f3\u30de\u30a6\u30b9\u3092\u7528\u3044\u305f\u624b\u6cd5\u3092\u7528\u3044\u3066\u3044\u307e\u3059(Sasaki et al., J Reprod Dev 2021)\u3002\u307e\u305f\u3001in vitro\u304b\u3089\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u3068\u3057\u3066\u306f\u3001\u79c1\u305f\u3061\u304c\u958b\u767a\u3057\u305f\u4f53\u5916\u5375\u6210\u9577\u57f9\u990a\u6cd5\u3092\u7528\u3044\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u57f9\u990a\u6cd5\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u8a66\u9a13\u7ba1\u5185\u3067\u5375\u5b50\u304c\u3067\u304d\u308b\u69d8\u5b50\u3092\u7d4c\u6642\u7684\u306b\u89b3\u5bdf\u3057\u306a\u304c\u3089\u89e3\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f(Morohaku et al., Proc Natl Acad Sci USA 2016)\u3002\u3053\u308c\u3089\u306e\u624b\u6cd5\u3092\u99c6\u4f7f\u3057\u3001\u300e\u5375\u5b50\u304c\u51fa\u6765\u4e0a\u304c\u308b\u4ed5\u7d44\u307f\u300f\u3092\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u306a\u89b3\u70b9\u304b\u3089\u89e3\u660e\u3057\u3066\u3044\u304d\u305f\u3044\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n
<\/p>\n
\u8fd1\u5e74\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u306e\u767a\u9054\u306f\u8457\u3057\u304f\u3001\u3081\u307e\u3050\u308b\u3057\u3044\u30b9\u30d4\u30fc\u30c9\u3067\u69d8\u3005\u306a\u6280\u8853\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306f\u3072\u3068\u3064\u306e\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3059\u308b\u306e\u306b\u5e74\u5358\u4f4d\u306e\u6642\u9593\u3092\u8981\u3057\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u7528\u3044\u308c\u3070\u308f\u305a\u304b\u6570\u30f6\u6708\u3067\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u4f5c\u51fa\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u4f34\u3044\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u7528\u3044\u305f\u7814\u7a76\u306e\u9700\u8981\u306f\u3053\u308c\u307e\u3067\u306b\u5897\u3057\u3066\u9ad8\u307e\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u751f\u7269\u8cc7\u6e90\u30bb\u30f3\u30bf\u30fc\u3067\u306fCRISPR-Cas\u3092\u7528\u3044\u3066\u69d8\u3005\u306a\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u4f5c\u51fa\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u5358\u7d14\u306a\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3060\u3051\u3067\u306a\u304f\u3001\u77ed\u3044\u914d\u5217\u3092DNA\u4e0a\u306e\u7279\u5b9a\u306e\u90e8\u4f4d\u306b\u633f\u5165\u3059\u308b\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a4\u30f3\u6280\u8853\u3084\u3001\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a4\u30f3\u6280\u8853\u3092\u5fdc\u7528\u3057\u3066\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u7d30\u80de\u7a2e\u7279\u7570\u7684\u306b\u963b\u5bb3\u3059\u308b\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u6cd5\u3082\u958b\u767a\u3057\u3066\u304d\u307e\u3057\u305f(Sasaki et al., J Reprod Dev 2021)\u3002\u3053\u308c\u3089\u306e\u6280\u8853\u306f\u907a\u4f1d\u5b50\u6a5f\u80fd\u3092\u3088\u308a\u8a73\u7d30\u306b\u89e3\u660e\u3059\u308b\u306e\u306b\u975e\u5e38\u306b\u6709\u52b9\u306a\u30c4\u30fc\u30eb\u3067\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u6280\u8853\u958b\u767a\u3092\u63a8\u9032\u3057\u3001\u533b\u5b66\u30fb\u751f\u7269\u5b66\u7814\u7a76\u3078\u306e\u8ca2\u732e\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n
CRISPR-Cas9\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u30de\u30a6\u30b9\u4f5c\u51fa\u306e\u5171\u540c\u7814\u7a76\u3092\u52df\u96c6\u3057\u3066\u3044\u307e\u3059\u3002\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u306f\u3058\u3081\u3068\u3057\u305f\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u4f5c\u88fd\u3092\u304a\u8003\u3048\u306e\u65b9\u306f\u305c\u3072\u304a\u58f0\u304c\u3051\u304f\u3060\u3055\u3044\u3002<\/p>\n
<\/p>\n
\u30d2\u30c8\u306e\u751f\u6b96\u88dc\u52a9\u533b\u7642\u3078\u306e\u8ca2\u732e\u3092\u6700\u7d42\u76ee\u7684\u3068\u3057\u3066\u3001\u30de\u30a6\u30b9\u521d\u671f\u80da\u306e\u64cd\u4f5c\u6280\u8853\u306e\u5411\u4e0a\u65b9\u6cd5\u3092\u7814\u7a76\u3057\u3066\u3044\u307e\u3059\u3002\u73fe\u5728\u306f\u3001\u30de\u30a6\u30b9\u3067\u306e\u4f53\u5916\u53d7\u7cbe\u3001\u53d7\u7cbe\u5375\u51cd\u7d50\u3001\u7cbe\u5b50\u51cd\u7d50\u304c\u5b89\u5b9a\u3057\u3066\u5b9f\u65bd\u3067\u304d\u3066\u3044\u307e\u3059\u3002\u672c\u6280\u8853\u306f\u3001\u8cb4\u91cd\u306a\u30de\u30a6\u30b9\u7cfb\u7d71\u306e\u4fdd\u5b58\u3084\u9069\u5207\u306a\u52d5\u7269\u98fc\u80b2\u74b0\u5883\u7dad\u6301\u306b\u5f79\u7acb\u3061\u307e\u3059\u3002\u3055\u3089\u306a\u308b\u9ad8\u52b9\u7387\u5316\u306b\u3088\u308a\u52d5\u7269\u5b9f\u9a13\u306b\u304a\u3051\u308b3R\u3078\u3082\u8ca2\u732e\u3057\u307e\u3059\u3002<\/p>\n
2018\u5e74\u3001\u79c1\u9054\u306f\u77ed\u3044\u30b9\u30c8\u30ed\u30fc\u306b\u30de\u30a6\u30b9\u7cbe\u5b50\u3092\u51cd\u7d50\u4fdd\u5b58\u3059\u308b\u6280\u8853\uff08ST\u6cd5\uff09\u3092\u958b\u767a\u3057\u307e\u3057\u305f(Kaneko R et al, 2018)\u3002\u672c\u6cd5\u306b\u3088\u308a\u3001\u51cd\u7d50\u7cbe\u5b50\u306e\u53ce\u7d0d\u30b9\u30da\u30fc\u30b9\u304c\u683c\u6bb5\u306b\u5c11\u306a\u304f\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n
<\/p>\n
\u4e3b\u306a\u7814\u7a76\u696d\u7e3e\uff1a<\/span><\/strong><\/pre>\n2021
\nKeisuke Sasaki, Saaya Takaoka, Yayoi Obata.\u00a0Oocyte-specific gene knockdown by intronic artificial microRNAs driven by Zp3<\/em> transcription in mice.\u00a0J Reprod Dev, 2021, 67: 229\u2013234<\/p>\nRyosuke Kaneko (Corresponding author), Toshie Kakinuma, Sachiko Sato, Atsushi Jinno-Oue.\u00a0Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling.\u00a0J Reprod Dev, \u00a02021, 67: 235\u2013239<\/p>\n
2019
\nKeisuke Sasaki, Satoshi Hara, Reina Yamakami, Yusuke Sato, Saki Hasegawa, Tomohiro Kono, Kanako Morohaku, Yayoi Obata.\u00a0Ectopic expression of DNA methyltransferases DNMT3A2 and DNMT3L leads to aberrant hypermethylation and postnatal lethality in mice.\u00a0Mol Reprod Dev, 2019, 86: 614\u2013623<\/p>\n
2018
\nKaneko R (corresponding author), Kakinuma T, Sato S, Jinno-Oue A.\u00a0Freezing sperm in short straws reduces storage space and allows transport in dry ice.\u00a0J Reprod Dev, 2018, 64:541-545<\/p>\n
Ryosuke Kaneko (corresponding author), Yusuke Takatsuru, Ayako Morita, Izuki Amano, Asahi Haijima, Itaru Imayoshi, Nobuaki Tamamaki, Noriyuki Koibuchi,Masahiko Watanabe, Yuchio Yanagawa.\u00a0Inhibitory neuron-specific Cre-dependent red fluorescent labeling using VGAT BAC-based transgenic mouse lines with identified transgene integration site.\u00a0Journal of Comparative Neurology, 2018, 526(3): 373-396<\/p>\n
2016
\nKaneko R, Sato A, Hamada S, Yagi T, Ohsawa I, Ohtsuki M, Kobayashi E, Hirabayashi M, Murakami T. Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT). Transgenic Res, 2016, 25(4):413-24<\/p>\n
Kanako Morohaku, Ren Tanimoto, Keisuke Sasaki, Ryouka Kawahara-Miki, Tomohiro Kono, Katsuhiko Hayashi, Yuji Hirao, Yayoi Obata.\u00a0Complete in vitro generation of fertile oocytesfrom mouse primordial germ cells.\u00a0Proc Natl Acad Sci U S A, 2016, 113: 9021\u20136<\/p>\n
2014
\nRyosuke Kaneko, Manabu Abe, Takahiro Hirabayashi, Arikuni Uchimura, Kenji Sakimura, Yuchio Yanagawa, Takeshi Yagi. Expansion of stochastic expression repertoire by tandem duplication in mouse Protocadherin-\u03b1 cluster. Scientific Reports, 2014, 4: 6263.<\/p>\n
Ryosuke Kaneko (corresponding author), Toshie Kakinuma, Sachiko Sato, Atsushi Jinno-Oue, and Hidekazu Hata. Littermate influence on infant growth in mice: Comparison of SJL\/J and ICR as cotransferred carrier embryos Experimental Animals, 2014, 63: 375-381<\/p>\n
Zhang Yue, Ryosuke Kaneko, Yuchio Yanagawa, and Yasuhiko Saito The vestibulo- and preposito-cerebellar cholinergic neurons of a ChAT-tdTomato transgenic rat exhibit heterogeneous firing properties and the expression of various neurotransmitter receptors Eur J Neurosci, 2014, 39: 1294-1313<\/p>\n
2012
\nKeizo Hirano, Ryosuke Kaneko (co-first author), Takeshi Izawa, Masahumi Kawaguchi, Takashi Kitsukawa, Takeshi Yagi Single-neuron diversity generated by Protocadherin-\u03b2 cluster in mouse central and peripheral nervous systems. Front Mol Neurosci. 2012, 5:90.<\/p>\n","protected":false},"excerpt":{"rendered":"
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